Accès gratuit
Numéro
Med Sci (Paris)
Volume 31, Numéro 1, Janvier 2015
Page(s) 84 - 92
Section M/S Revues
DOI https://doi.org/10.1051/medsci/20153101017
Publié en ligne 6 février 2015
  1. Sawyers CL. The cancer biomarker problem. Nature 2008 ; 452 : 548–552. [CrossRef] [PubMed]
  2. Morrison TB, Weis JJ, Wittwer CT. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques 1998 ; 24 : 954–962. [PubMed]
  3. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5’-3’ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991 ; 88 : 7276–7280. [CrossRef]
  4. Thierry AR, Mouliere F, El Messaoudi S, et al. Clinical validation of the detection of KRAS and BRAF mutations from circulating tumor DNA. Nat Med 2014 ; 20 : 430–435. [CrossRef] [PubMed]
  5. Sykes PJ, Neoh SH, Brisco MJ, et al. Quantitation of targets for PCR by use of limiting dilution. Biotechniques 1992 ; 13 : 444–449. [PubMed]
  6. Vogelstein B, Kinzler KW, Digital PCR. Proc Natl Acad Sci USA 1999 ; 96 : 9236–9241. [CrossRef]
  7. Hindson BJ, Ness KD, Masquelier DA, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 2011 ; 83 : 8604–8610. [CrossRef] [PubMed]
  8. Dingle TC, Sedlak RH, Cook L, Jerome KR. Tolerance of droplet-digital PCR versus real-time quantitative PCR to inhibitory substances. Clin Chem 2013 ; 59 : 1670–1672. [CrossRef] [PubMed]
  9. Baker M.. Digital PCR hits its stride. Nat Methods 2012 ; 9 : 541–544. [CrossRef]
  10. Baret JC, Taly V, Ryckelynck M, et al. Gouttes et émulsions : criblage à très haut débit en biologie. Med Sci (Paris) 2009 ; 25 : 627–632. [CrossRef] [EDP Sciences] [PubMed]
  11. Morrison T, Hurley J, Garcia J, et al. Nanoliter high throughput quantitative PCR. Nucleic Acids Res 2006 ; 34 : e123. [CrossRef] [PubMed]
  12. Thorsen T, Maerkl SJ, Quake SR. Microfluidic large-scale integration. Science 2002 ; 298 : 580–584. [CrossRef] [PubMed]
  13. Warren L, Bryder D, Weissman IL, Quake SR. Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR. Proc Natl Acad Sci USA 2006 ; 103 : 17807–17812. [CrossRef]
  14. Yung TKF, Chan KCA, Mok TSK, et al. Single-molecule detection of epidermal growth factor receptor mutations in plasma by microfluidics digital PCR in non-small cell lung cancer patients. Clin Cancer Res 2009 ; 15 : 2076–2084. [CrossRef] [PubMed]
  15. Henríquez-Hernández LA, Valenciano A, Herrera-Ramos E, et al. High-throughput genotyping system as a robust and useful tool in oncology: experience from a single institution. Biologicals 2013 ; 41 : 424–429. [CrossRef] [PubMed]
  16. Hudson J, Duncavage E, Tamburrino A, et al. Overexpression of miR-10a and miR-375 and downregulation of YAP1 in medullary thyroid carcinoma. Exp Mol Pathol 2013 ; 95 : 62–67. [CrossRef] [PubMed]
  17. Shen F, Du W, JE Kreutz JE, et al. Digital PCR on a SlipChip. Lab Chip 2010 ; 10 : 2666–2672. [CrossRef] [PubMed]
  18. Williams R, Peisajovich SG, Miller OJ, et al. Amplification of complex gene libraries by emulsion PCR. Nat Methods 2006 ; 3 : 545–550. [CrossRef] [PubMed]
  19. Huggett JF, Foy CA, Benes V, et al. The digital MIQE guidelines: minimum information for publication of quantitative digital PCR experiments. Clin Chem 2013 ; 59 : 892–902. [CrossRef] [PubMed]
  20. Theberge AB, Courtois F, Schaerli Y, et al. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew Chemie 2010 ; 49 : 5846–5868. [CrossRef]
  21. Dressman D, Yan H, Traverso G, et al. Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Proc Natl Acad Sci USA 2003 ; 100 : 8817–8822. [CrossRef]
  22. Diehl F, Li M, Dressman D, et al. Detection and quantification of mutations in the plasma of patients with colorectal tumors. Proc Natl Acad Sci USA 2005 ; 102 : 16368–16373. [CrossRef]
  23. Taly V, Pekin D, El Abed A, Laurent-Puig P. Detecting biomarkers with microdroplet technology. Trends Mol Med 2012 ; 18 : 405–416. [CrossRef] [PubMed]
  24. Whitesides GM. The origins and the future of microfluidics. Nature 2006 ; 442 : 368–373. [CrossRef] [PubMed]
  25. The SY, Lin R, Hung LH, Lee AP. Droplet microfluidics. Lab Chip 2008 ; 8 : 198–220. [CrossRef] [PubMed]
  26. Baret JC. Surfactants in droplet-based microfluidics. Lab Chip 2012 ; 12 : 422–433. [CrossRef] [PubMed]
  27. Baret JC, Beck Y, Billas-Massobrio I, et al. Quantitative cell-based reporter gene assays using droplet-based microfluidics. Chem Biol 2010 ; 17 : 528–536. [CrossRef] [PubMed]
  28. Beer NR, Wheeler EK, Lee-houghton L, et al. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets. Anal Chem 2008 ; 80 : 1854–1858. [CrossRef] [PubMed]
  29. Hatch AC, Fisher JS, Tovar AR, et al. 1-Million droplet array with wide-field fluorescence imaging for digital PCR. Lab Chip 2011 ; 11 : 3838–3845. [CrossRef] [PubMed]
  30. Kiss MM, Ortoleva-Donnelly L, Beer NR, et al. High-throughput quantitative polymerase chain reaction in picoliter droplets. Anal Chem 2008 ; 80 : 8975–8981. [CrossRef] [PubMed]
  31. Pekin D, Skhiri Y, Baret JC, et al. Quantitative and sensitive detection of rare mutations using droplet-based microfluidics. Lab Chip 2011 ; 11 : 2156–2166. [CrossRef] [PubMed]
  32. Taly V, Pekin D, Benhaim L, et al. Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients. Clin Chem 2013 ; 59 : 1722–1731. [CrossRef] [PubMed]
  33. Diaz LA, Bardelli A. Liquid biopsies: genotyping circulating tumor DNA. J Clin Oncol 2014 ; 32 : 579–586. [CrossRef] [PubMed]
  34. Nixon G, Garson JA, Grant P, et al. Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus. Anal Chem 2014 ; 86 : 438794. [CrossRef]
  35. Sedlak RH, Cook L, Cheng A, et al. Evaluation of the clinical utility of droplet digital PCR for human cytomegalovirus. J Clin Microbiol 2014 ; 52 : 6–11. [CrossRef]
  36. Lun FMF, Chiu RWK, Allen Chan KC, et al. Microfluidics digital PCR reveals a higher than expected fraction of fetal DNA in maternal plasma. Clin Chem 2008 ; 54 : 1664–1672. [CrossRef] [PubMed]
  37. Fan HC, Quake SR. Detection of aneuploidy with digital polymerase chain reaction. Anal Chem 2007 ; 79 : 7576–7579. [CrossRef] [PubMed]
  38. Lun FMF, Tsui NBY, Chan KCA, et al. Noninvasive prenatal diagnosis of monogenic diseases by digital size selection and relative mutation dosage on DNA in maternal plasma. Proc Natl Acad Sci USA 2008 ; 105 : 19920–19925. [CrossRef]
  39. Fallah-Araghi A, Baret JC, Ryckelynck M, Griffiths AD. A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution. Lab Chip 2012 ; 12 : 882–891. [CrossRef] [PubMed]
  40. Simi L, Pratesi N, Vignoli M, et al. High-resolution melting analysis for rapid detection of KRAS, BRAF, and PIK3CA gene mutations in colorectal cancer. Am J Clin Pathol 2008 ; 130 : 247–253. [CrossRef] [PubMed]
  41. Weichert W, Schewe C, Lehmann A, et al. KRAS genotyping of paraffin-embedded colorectal cancer tissue in routine diagnostics. J Mol Diagnostics 2010 ; 12 : 35–42. [CrossRef]
  42. Lièvre A, Bachet J-B, Boige V, et al. KRAS mutations as an independent prognostic factor in patients with advanced colorectal cancer treated with cetuximab. J Clin Oncol 2008 ; 26 : 374–379. [CrossRef] [PubMed]
  43. Caen O, Nizard P, Garrigou S, et al. Apport de la PCR digitale pour la détection quantitative d’ADN tumoral circulant. Med Sci (Paris) 2015 ; 31 (sous presse). [CrossRef] [EDP Sciences] [PubMed]

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