UNBS5162 promotes HCT116 cells apoptosis. A. HCT116 cells cultured in presence of UNBS5162 or DMSO were labeled with Annexin V/FITC and PI solution. Results from flow cytometry analysis (Flowjo software) showed that the percentage of apoptotic cells was 22.7%, while only 5.03% in the control group (NC). B. The expression levels of Bcl-2, Bax and active caspase-3 proteins were measured using Western-blot analyses after incubation with UNBS5162 or DMSO for 24 h. C. Three experiments were repeated to confirm the results, P<0.05 compared with the control group (NC).