miR-25 targets the RNA-binding protein 47. (A) Luciferase-based reporter gene assay detects RBM47 inhibition by miR-25 through the targeting of its promoter. (B) miR-25 inhibits RBM47 protein expression in M14 cells. (C) CKK-8 assay of M14 cell proliferation after co-transfection of miR-25 plasmid or NC plasmid with RBM47 expression plasmid or empty plasmid used as a control. (D) Transwell migration assay of M14 cells after co-transfection of miR-25 plasmid or NC plasmid with RBM47 expression plasmid or empty plasmid used as a control. 10% FBS was used as the chemoattractant. ^*P < 0.05. P values were determined using Student’s t-tests.