Med Sci (Paris)
Volume 34, October 2018Cancer biomarkers
|Page(s)||59 - 65|
|Published online||07 November 2018|
miR-25 Promotes Melanoma Progression by regulating RNA binding motif protein 47
Department of Dermatology, 404 Hospital of People’s Liberation Army, No.8 of Baoquan Street, Huancui District, Weihai, 264200, Shandong Province, China
* Corresponding author, Qun-Qun Jiang email@example.com
Melanoma is the most aggressive skin cancer, and accounts for the major part of skin cancer-related deaths in the world. In addition, the underlying mechanism of tumor progression in melanoma remains far from being elucidated. In this study, we have evaluated the function of miR-25 in melanoma. First, we examined the expression of miR-25 in four melanoma cell lines (A875, MV3, M14 and uacc-257) and in a normal melanocyte cell line (HEM-a). Then, we overexpressed miR-25 in M14 cells. Our results show that miR-25 promotes M14 cell proliferation and migration. We found that miR-25 up-regulates the PI3K/Akt/mTOR signaling pathway in these tumor cells. Furthermore, a luciferase-based reporter gene assay showed that miR-25 could directly target the RNA-binding motif protein 47 (RBM47). Taken together, our findings suggest that RBM47 is a promising target for the treatment of melanoma.
Key words: melanoma cell / migration / miR-25 / RBM47 / proliferation
© 2018 médecine/sciences – Inserm
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