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Tableau I
Différentes méthodes de microscopie employées dans cette étude et leur limites.
| Microscopie | Cellule | Champ de vision | Fixation | Taille de l’échantillon | Taille de pixel (nm3) (xyz) |
|---|---|---|---|---|---|
| SXT - capillaire | Cellule en suspension | Cellule entière | Vitrification | Multiples cellules | 25 x 25 x 25 |
| SXT - grille | Cellule adhérente | 1/3 de la cellule | Vitrification | Multiples cellules | 12 x 12 x 12 |
| SBF-SEM | Cellule adhérente | Cellule entière | Fixation chimique | Une seule cellule | 8 x 8 x 40 |
| FIB-SEM | Cellule adhérente | Partie de la cellule | Fixation chimique | Plusieurs mitochondries | 5 x 5 x 5 |
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