UNBS5162 inhibits the activation of the PI3K/Akt signaling pathway in HCT116 cells. A. Western-blot analysis of proteins involved in the PI3K/Akt signaling pathway in HCT116 cells cultured in presence of UNBS5162 or DMSO (NC). B. The expression levels of proteins (indicated in the ordinate legends) detected by Western-blot experiments performed in triplicates were analyzed using the QUANTITY ONE software, P<0.05 compared with the control group (NC).