Four shRNAs inhibited the filaggrin expression both at the mRNA and protein levels 72 h after shRNA transfection into NHEKs. A. Expression of filaggrin mRNA after transfection with each kind of shRNA, compared to the control (NC). B. Western blotting of filaggrin after NHEKs transfection with each kind of shRNA. C. Semi-quantitative analysis of filaggrin protein levels for each kind of shRNA transfection into NHEKs, compared to the control group (NC). n=3, ^** P<0.001.