NP-2143 treatment induces apoptosis of M14 cells and regulates the expression of apoptosis‑related proteins. A. Flow cytometry staining reveals a significant shift in Annexin V-FITC-positive cells after NPS-2143 treatment of M14 cells. B. The percentage of apoptotic cells is shown (^* P < 0.05). C. M14 cell lysates were separated by SDS-PAGE and analyzed by western blots with the indicated antibodies. GAPDH was used as a loading control. D. The values of the band intensity represent the densitometric estimation of each band normalized to GAPDH (^* P < 0.05).