Overexpression of RBM10 induces U2OS osteosarcoma cell apoptosis via promoting TNF- a production. (A) U2OS cells were transfected with pcDNA3.1-RBM10. Cells were then harvested, stained with AnnexinV and PI and analyzed by flow cytometry. The results are shown as mean ± S.D. * P<0.05. (B) RBM10-overexpressed U2OS cells (RBM10) and negative control cells (NC) were lysed and subjected to SDS-PAGE and Western Blotting was performed to detect the expression of Bcl-2 and capsase-3. Data are representative of three independent experiments. * P<0.05. (C) RNA was extracted from RBM10-overexpressing U2OS cells (RBM10) and negative control cells (NC). The expression of TNF-α was detected by qPCR. Results are shown as mean ± S.D. * P<0.05. (D) U2OS cells were transfected with pcDNA3.1-RBM10. Supernatants were harvested and TNF-α production was detected by ELISA. The results are shown as mean ± S.D. * P<0.05.