Overexpression of RBM10 suppresses growth of U2OS osteosarcoma cells. (A) U2OS cells were transfected with pcDNA3.1-RBM10. RNA was then extracted and the expression of RBM10 was assessed by qPCR. Data are shown as mean ± S.D. of three independent experiments. * P<0.05. (B) RBM10-overexpressed U2OS cells (RBM10) and negative control cells (NC) were lysed and subjected to SDS-PAGE and Western Blotting was performed to detect the expression of RBM10. Data are representative of three independent experiments. * P<0.05. (C) RBM10-overexpressed U2OS cells (RBM10) and negative control cells (NC) were seeded in 96-well plates. CCK-8 assay was performed every day for 4 days. The experiment was performed in quintuplicate. The results are shown as mean ± S.D. * P<0.05.