A strong expression of miR-25 is associated with an increase of melanoma M14 cell proliferation. (A) CKK-8 assay of M14 cells proliferation after miR-25 plasmid or NC plasmid transfection. (B) Colony formation assay of M14 cells transfected with NC or miR-25 plasmids. Cells were fixed and stained with Giemsa. One representative experiment out of three is shown. * P<0.05. P values were determined using Student’s t-tests.