L’activation/dégradation protéasomique des GTPases Rho par CNF1 confère des capacités invasives aux E. coli uropathogènes

Luce Landraud; Anne Doye; Caroline Buisson-Touati; Patrice Boquet; Emmanuel Lemichez

doi:doi:10.1051/medsci/2003194403

Accès gratuit

Numéro		Med Sci (Paris) Volume 19, Numéro 4, Avril 2003


Page(s)		403 - 405
Section		Le Magazine : Nouvelles
DOI		http://dx.doi.org/10.1051/medsci/2003194403
Publié en ligne		15 April 2003

Warren JW. Clinical presentation and epidemiology of urinary tract infections. In: Mobley HTL, Warren JW, eds. Urinary tract infections. Molecular pathogenesis and clinical management. Washington: American Society for Microbiology, 1996 : 3–27.
Landraud L, Gauthier M, Fosse T, Boquet P. Frequency of Escherichia coli strains producing the cytotoxic necrotizing factor (CNF1) in nosocomial urinary tract infections. Lett Appl Microbiol 2000; 30: 213–6.
Boquet P, Lemichez E. Bacterial virulence factors targeting Rho GTPases: parasitism or symbiosis. Trends Cell Biol 2003 (sous presse).
Lemichez E, Flatau G, Bruzzone M, Boquet P, Gauthier M. Molecular localisation of the Escherichia coli cytotoxic necrotizing factor CNF1 cell-binding and catalytic domains. Mol Microbiol 1997; 97: 1061–70.
Contamin S, Galmiche A, Doye A, Flatau G, Benmerah A, Boquet P. The p21 Rhoactivating toxin cytotoxic necrotizing factor 1 is endocytosed by a clathrinindependent mechanism and enters the cytosol by an acidic-dependent membrane translocation step. Mol Biol Cell 2000; 11: 1775–87.
Pei S, Doye A, Boquet P. Mutation of specific acidic residues of the CNF1 T domain into lysine alters cell membrane translocation of the toxin. Mol Microbiol 2001; 41: 1237–47.
Flatau G, Lemichez E, Gauthier M, et al. Toxininduced activation of the G protein p21 Rho by deamidation of glutamine. Nature 1997; 387: 729–33.
Hall A. Rho GTPases and the actin cytoskeleton. Science 1998; 279: 509–14.
Caron E, Hall A. Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases. Science 1998; 282: 1717–21.
Doye A, Mettouchi A, Bossis G, et al. CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion. Cell 2002; 111: 553–64.
Rihet S, Vielh P, Camonis J, Goud B, Chevillard S, de Gunzburg J. Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors. J Cancer Res Clin Oncol 2001; 127: 733–8.
Galàn JE, Zhou D. Striking a balance: modulation of the actin cytoskeleton by Salmonella. Proc Natl Acad Sci USA 2000; 97: 8754–61.

[1] Warren JW. Clinical presentation and epidemiology of urinary tract infections. In: Mobley HTL, Warren JW, eds. Urinary tract infections. Molecular pathogenesis and clinical management. Washington: American Society for Microbiology, 1996 : 3–27.

[2] Landraud L, Gauthier M, Fosse T, Boquet P. Frequency of Escherichia coli strains producing the cytotoxic necrotizing factor (CNF1) in nosocomial urinary tract infections. Lett Appl Microbiol 2000; 30: 213–6.

[3] Boquet P, Lemichez E. Bacterial virulence factors targeting Rho GTPases: parasitism or symbiosis. Trends Cell Biol 2003 (sous presse).

[4] Lemichez E, Flatau G, Bruzzone M, Boquet P, Gauthier M. Molecular localisation of the Escherichia coli cytotoxic necrotizing factor CNF1 cell-binding and catalytic domains. Mol Microbiol 1997; 97: 1061–70.

[5] Contamin S, Galmiche A, Doye A, Flatau G, Benmerah A, Boquet P. The p21 Rhoactivating toxin cytotoxic necrotizing factor 1 is endocytosed by a clathrinindependent mechanism and enters the cytosol by an acidic-dependent membrane translocation step. Mol Biol Cell 2000; 11: 1775–87.

[6] Pei S, Doye A, Boquet P. Mutation of specific acidic residues of the CNF1 T domain into lysine alters cell membrane translocation of the toxin. Mol Microbiol 2001; 41: 1237–47.

[7] Flatau G, Lemichez E, Gauthier M, et al. Toxininduced activation of the G protein p21 Rho by deamidation of glutamine. Nature 1997; 387: 729–33.

[8] Hall A. Rho GTPases and the actin cytoskeleton. Science 1998; 279: 509–14.

[9] Caron E, Hall A. Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases. Science 1998; 282: 1717–21.

[10] Doye A, Mettouchi A, Bossis G, et al. CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion. Cell 2002; 111: 553–64.

[11] Rihet S, Vielh P, Camonis J, Goud B, Chevillard S, de Gunzburg J. Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors. J Cancer Res Clin Oncol 2001; 127: 733–8.

[12] Galàn JE, Zhou D. Striking a balance: modulation of the actin cytoskeleton by Salmonella. Proc Natl Acad Sci USA 2000; 97: 8754–61.

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